HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH CARPET SHELL CLAM (Ruditapes decussatus)

  • Aslı MURATLI Baskent University, Zübeyde Hanım Application and Research Center
  • Mehmet Rıza GEZEN Çanakkale Onsekiz Mart University
Keywords: Stomach, Carpet Shell Clam, Dardanelles, 8-hydroxy-2' -deoxyguanosine, Immunohistochemistry,

Abstract

Free radicals are produced in cells by cellular metabolism and exogenous agents. These specie reacts with biomolecules in cells, including DNA, proteins, lipids and carbonhydrates. The resulting oxidative damage to DNA, is implicated in mutagenesis, carcinogenesis, and aging. Heavy metals, industrial and household wastes and pesticides are threats for the aquatic ecosystem. Polluted water sources are streaming into the seas and cause pollution in these systems. Dardanelles is exposed to pollution from the Marmara and Black Sea. Our previous studies demonstrated that the water and mollusc from certain regions of the Dardanelles contained heavy metal salts. The purpose of the study is to demonstrate DNA damage and the histopathologic changes in the gastric tissues of rats which are fed with carpet shell clam that are collected from the Çardak Lagoon (Çanakkale, Turkey). Four groups of rats are included in the study, group 1 (n=6), control group fed with standard rat food, group 2 (n=6), 75%  carpet shell clam and 25% standard rat food daily, group 3 (n=6), 75% carpet shell clam and 25% standard rat food every two days, group 4 (n=6), 75%  carpet shell clam and 25% standard rat food every three days. After the routine histopathologic processing all gastric tissue samples are evaluated in terms of 8-hydroxy-2' -deoxyguanosine (8-OHdG) immunoreactivity with light microscopy and image analysis software. No histopathologic differences found in standard hematoxylin-eosin stained gastric tissue samples of the control group. Mucosal specimens of the second, third and fourth groups showed mild mononuclear inflammation in favor of chronic gastritis. In immunohistochemical staining, 8-OHdG immunoreactivity in gastric epithelial cells.  8-OHdG immunoreactivity was negative in stomach tissues in all groups.  There was no statistically significant difference between the groups that were fed every day, every other day and every three days with clam (p>0.05). The results of our studies showed that rats fed more with carpet shell clam could produce gastritis in the stomach.

Downloads

Download data is not yet available.

References

ATES, I., SUZEN, H.S., AYDIN, A., KARAKAYA, A., 2004, The Oxidative DNA Base Damage In Testes Of Rats After Intraperitoneal Cadmium Injection. Biometals, 17(4), 371-7. AVUNDUK, M.C., TAVLI, S., YOL, S., TAVLI, L., YAVUZ, A., GUNGOR, S., YILMAZ, O., 2000, Mide karsinomlarında hücre proliferasyon belirleyicisi olarak PCNA, Ki 67 ve agNOR kullanımı, Ankara Üniversitesi Tıp Fakültesi Mecmuası, 53(1), 11-15. BAKIR, K., KAPUCUOĞLU, N., BULBUL, H.D., 1996, PCNA (Proliferating Cell Nuclerar Antigene) immunohistokimyasal boyanması ve agNORs’un meme karsinomunda prognozu belirlemedeki rolü, Türk Patoloji Dergisi, 12(1), 35-38. BURCAK, G., ANDICAN, G., 2004, Oksidatif DNA Hasarı ve Yaşlanma, Cerrahpasa J Med, 35(4), 159-169. CLARKSON, T.W., 2002, The three modern faces of mercury, Environ Health Perspect, 110, 11-23. DE MARTINIS, B.S., DE LOURDES PIRES BIANCHI, M., 2002, Methodology For Urinary 8-hydroxy-2’-deoxyguanosine Analysis By HPLC With Electrochemical Detection, Pharmacol Res, 46(2), 129-31. DEMIR, N., GEZEN, M.R., BALLI, M., 2011, Çanakkale Boğazı’nın Karacaören Kıyısındaki Deniz Suyu ve Bazı Yumuşakçalarda (Bivalvia ve Gastropoda) Ağır Metal Düzeylerinin Araştırılması, Ekoloji 2011 Sempozyumu, 5-7 Mayıs, Düzce, 177. DIZDAROGLU, M., 1998, Facts About the Artifacts in the Measurement of Oxidative DNA Base Damage by Gas Chromatography-mass Spectrometry, Free Radic Res, 29(6), 551-63. FERNER, D.J., 2001, Toxicity, heavy metals, eMed J. 2(5), 1. FRAGA, C.G., SHINENAGA, M.K., PARK, J.W., DEGAN, P., AMES, B.N., 1990, Oxidative Damage to DNA During Aging: 8-hydroxy-2’-deoxyguanosine in Rat Organ DNA and Urine, Proc Natl Acad Sci USA, 87(12), 4533-7. GEZEN, M.R., DEMIR, N., ÇETIN, M., 2011, Çanakkale Boğazı’nın Umurbey Kıyısındaki Deniz suyu ve Bazı Yumuşakçalarda (Bivalvia ve Gastropoda) Ağır Metal Düzeylerinin Araştırılması. Ekoloji 2011 Sempozyumu, 5-7 Mayıs Düzce, 178. GEZEN, M.R., DEMIR, N., ÇETIN, M., BALLI, M., 2011, Çanakkale Boğazı’ndaki DenizKestanesi (Paracentrotus lividus)’ nin Ağır Metal Düzeyleri Üzerinde Bir Araştırma, Ekoloji 2011 Sempozyumu, 5-7 Mayıs Düzce, 176. GEZEN MR, 2017. Histopathological Research and Diagnosis of Important Paiting Methods, JSP, 1(1): 23-27. GULBAHAR, O., 2007, Protein Oksidasyonunun Mekanizması, Önemi ve Yaşlılıkla İlişkisi.Turkish Journal of Geriatrics, 10(1), 43-48, 2007. HALLIWELL, B., 2000, Why and How Should We Measure Oxidative DNA Damage in Nutritional Studies? How Far Have We Come? Am J Clin Nutr, 72(5), 1082-7.
IARC (International Agency for Research on Cancer) 1987, Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans: Arsenic and Arsenic Compounds (Group 1) Vol. 7. Lyon, France: World Health Organization; 100–106. IBRAHIM, D., FROBERG, B., WOLF, A., RUSYNIAK, D.E., 2006, Heavy metal poisoning: clinical presentations and pathophysiology, Clinics in Laboratory Medicine, 26(1), 67–97. JÄRUP, L., 2003, Hazards of heavy metal contamination, Br Med Bull, 68(1), 167–182. KULAKSIZ, G., SANCAR, A., 2007, Nükleotid Eksizyon Onarımı ve Kanser, Turk J Biochem, 32(3), 104-111. MANTOVANI, A., ALLAVENA, P., SICA, A., BALKWILL, F., 2008, Cancer-related İnflammation, Nature, 454, 436-44. NAVAS-ACIEN, A., SILBERGELD, E.K., SHARRETT, A.R., CALDERON-ARANDA, E., SELVIN, E., GUALLAR, E., 2005, Metals in urine and peripheral arterial disease, Environmental Health Perspectives, 113(2), 164–169. NIEBOER, E., TSUJI, L.J., MARTIN, I.D., LIBERDA, E.N., 2013, Human biomonitoring issues related to lead exposure. Environ Sci Process Impacts, 15: 1824-9. OZKURNAZ, G., DEMIR, N., 2012, Umurbey Bölgesi’nden (Çanakkale) Toplanan Mytilus galloprovincialis ve Pecten maximus Dokularında Ağır Metal Birikiminin İncelenmesi. 21. Ulusal Biyoloji Kongresi, İzmir, Türkiye, 3-7 Eylül, 880-881. PROZIALECK, W.C., EDWARDS, J.R., WOODS, J.M., 2006, The vascular endothelium as a target of cadmium toxicity, Life Sciences, 79(16), 1493–1506. SANCAR, A., LINDSEY-BOLTZ, L.A., UNSAL-KAÇMAZ, K., LINN, S., 2004, Moleculer mechanisms of mammalian DNA repair and the damage checkpoints, Annu Rev Biochem, 73, 39-85. SIOMEK, A., TUJAKOWSKI, J., GACKOWSKI, D., ROZALSKI, R., FOKSINSKI, M., DZIAMAN, T., ROSZKOWSKI, K., OLINSKI, R., 2006, Severe oxidatively damaged DNA after cisplatin treatment of cancer patients, Int J Cancer, 119(9), 2228-30. TOSUN, E., TOSUN, M., AVUNDUK, M.C., 2006, Hücre Çoğalma Belirleyicilerinin Meme İnfiltratif Duktal Karsinomlarında Progrostik Amaçlı Kullanımı, Optimal Tıp Dergisi, 19(2), 27-40. WILLIAMS, G.M., JEFFREY, A.M., 2000, Oxidative DNA Damage: Endogenous and Chemically Induced, Regul Toxicol Pharmacol, 32(3), 283-92.
Published
2018-07-31
How to Cite
MURATLI, A., & GEZEN, M. (2018). HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH CARPET SHELL CLAM (Ruditapes decussatus). JOURNAL OF SCIENTIFIC PERSPECTIVES, 2(3), 115-124. https://doi.org/10.26900/jsp.2018342250
Section
Health Science